Journal: ACS Applied Materials & Interfaces

Article Title: Silver Nitrate and Bioactive Glass Containing Chitosan Coatings: Comparison of Antimicrobial and Antiviral Properties

doi: 10.1021/acsami.6c00215

Figure Lengend Snippet: Metallic air filters coated with chitosan/BG or chitosan/AgNO 3 showed no cytotoxicity on any tested cell lines. Cytotoxicity assessment was performed on (a) MRC-5 for HCoV-OC43, (b) HeLa 24 h for HRV-A1, (c) HeLa 96 h for AdV-5, and (d) MDCK for IFV-A H3N2. The cytotoxic effect of coatings on host cells was analyzed via optical microscope observation as reported in ISO guideline (a–d). Magnification, 400×. DMEM, culture medium; DMEM 2%, culture medium supplemented with 2% of serum; pure, pure wash-out solution; 1:10, 1:10 diluted wash-out solution.

Article Snippet: Human lung fibroblasts (MRC-5) (ATCC CCL-171), human cervix adenocarcinoma cells (HeLa) (ATCC CCL-2), and Madine-Darby canine kidney epithelial cells (MDCK) (ATCC CCL-34) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Sigma-Aldrich) supplemented with heat-inactivated, 10% (v/v) fetal bovine serum (FBS; Sigma-Aldrich) in a humidified 5% CO 2 incubator.

Techniques: Microscopy